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Medi-gel
Medi-gel (medical gel) is an all-purpose medicinal salve combining an anaesthetic and clotting agent used by paramedics, EMTs, and military personnel, produced by the Sirta Foundation. It heals various wounds and ailments, instantly sealing injuries against infection and allowing for rapid healing by having the gel grip tight to flesh until subjected to a frequency of ultrasound. It is sealable against liquids - most notably blood - as well as contaminants and gases. Technically its properties make it illegal under genetic modification laws, but the restriction, in this case, is ignored because of the product's beneficial properties.
Mass Effect
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Medi-gel can be gained from looting or as a reward from NPCs. It can also be replenished from aid stations, from the SSV Normandy's medical bay, at the Med Clinic on the Citadel, or (if available) the apartment on Intai'sei. At various shops one can purchase upgrades that allow more medi-gel to be carried (up to a maximum of 10 units).
Using Medi-gel involves a 60 second cooldown period which can be shortened with high-grade omni-tools and / or the Medicine talent. It heals all wounded squad members for a base value of 40 health. The amount of health restored on medi-gel can be increased with the First Aid talent, and the Medic specialization allows medi-gel to revive fallen squad members.
Mass Effect 2
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In Mass Effect 2, Medi-gel is no longer used in the same way. A new self-healing system (see Codex "Body Armor" entry), managed by your armor suit microframe computers, automatically uses small quantities of Medi-gel when required in order to slowly regenerate your squad members' health (as long as they are not taking damage). Massive doses (corresponding to the Medi-gel units Shepard can acquire) can be injected through the revamped Unity power. Medi-gel initially is used only to revive fallen squadmates, but research upgrades allow Medi-gel to be used to restore the health and shields of all squadmates as well.
Medi-gel is acquired through opening aid containers or wall-mounted aid stations. Shepard can research upgrades which allow him or her to carry additional Medi-gel units. Shepard can initially carry up to 3 Medi-gel units; this can be upgraded to a maximum of 9 Medi-gel units. If Shepard opens a Medi-gel container while he or she is carrying the maximum number of Medi-gel units, the Medi-gel will be salvaged for a total of 100 credits.
Each time a Medi-gel is utilized to revive fallen squadmates, one is used per fallen squadmate. However, if both squadmates are knocked out and the player only has 1 Medi-gel remaining, the use of that single Medi-gel will revive both squadmates at the same time.
Mass Effect 3
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Similar to how it was used in Mass Effect 2, Medi-gel in Mass Effect 3 is used to revive squadmates and to restore health to Shepard in the single player campaign. Shepard only regenerates missing health from the current health bar they are on.
Medi-gel is no longer directly necessary to revive injured squadmates, as Shepard can go to their location and revive them manually by standing in place near the downed squadmate and activating the revive. This method is exactly the same as the multiplayer revival method.
Instead of getting credits for surplus medi-gel found on missions, however, Shepard now receives bonus experience points. The amount received corresponds to the difficulty level at that point: 25 XP for Easy/Casual, 50 XP for Normal, 100 XP for Hardcore, and 150 XP for Insanity per medi-gel. Med-kits contain 2 medi-gels, while med-stations may contain more.
Medi-gel in multiplayer is available in a limited capacity to self-revive after being downed, but cannot be used if a Phantom, Atlas, Brute, or Banshee performs their combat execution maneuver. A maximum of two medi-gel per player per game is allotted for balance, however you can get upgrades from the shop that allow you to carry more as with all the in game usables.